Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions.

نویسندگان

  • K G Gasaryan
  • V Z Tarantul
  • Y N Baranov
  • L Y Frolova
  • L L Kisselev
چکیده

The kinetics of hybridization of pigeon globin messenger RNA with complementary cDNA synthesized by means of AMV reverse transcriptase is complex. Addition of poly A or poly U in excess to the reaction mixture normalized the kinetics. It is concluded that association of the complementary homopolymeric regions of mRNA and cDNA accelerates the complex formation between heteropolymeric sequences in a fraction of the molecules.

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عنوان ژورنال:
  • Nucleic acids research

دوره 2 7  شماره 

صفحات  -

تاریخ انتشار 1975